An Improved Method for the Preparation of the Four Ribonucleic Acids of Cowpea Chlorotic Mottle Virus
نویسندگان
چکیده
The four components of the genome of cowpea chlorotic mottle virus have been prepared in a highly active and highly purified state by a method based on their resolution by polyacrylamide gel electrophoresis. Activity and purity have been confirmed by gel electrophoresis under denaturing and non-denaturing conditions, infectivity tests on whole plants, translation in an mRNA dependent rabbit reticulocyte lysate and fingerprinting of Tj ribonuclease digests after labelling with 32P at the 5' ends of the oligonucleotide products. The quality of the RNAs obtained was superior to those previously obtained by zonal centrifugation but only comparatively small batches of whole RNA can be handled with ease.
منابع مشابه
A method for the purification of large quantities of biologically active ribonucleic acid components from cowpea chlorotic mottle virus, a multicomponent plant virus.
Cowpea chlorotic mottle virus RNA has been prepared in comparatively high yield (at least 50%) by a modified phenol extraction method. The preparation, which has high biological activity, has been resolved into four components by zonal centrifugation on a 15-40% (w/v) sucrose density gradient. The components obtained have been tested for biological activity against whole plants and plant protop...
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